Coding

Part:BBa_K2201010:Design

Designed by: Christopher M. Whitford   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)


Construct comprised of PtNTT2, lacO and Cas9


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3356
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3356
    Illegal NheI site found at 3115
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3356
    Illegal BamHI site found at 5394
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3356
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3356
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Construct comprised of PtNTT2, lacO and Cas9


Source

Construct comprised of PtNTT2, lacO and Cas9

References